Improving Metabolic Engineering and Characterization of Clostridium Thermocellum for Improved Cellulosic Ethanol Production

Improving Metabolic Engineering and Characterization of Clostridium Thermocellum for Improved Cellulosic Ethanol Production
Author: Beth Alexandra Papanek
Publisher:
Total Pages: 91
Release: 2016
Genre: Biomass energy
ISBN:


Download Improving Metabolic Engineering and Characterization of Clostridium Thermocellum for Improved Cellulosic Ethanol Production Book in PDF, Epub and Kindle

Biofules are an important option for humanity to move away from its dependence on fossil fuels. Transitioning from food crops to lignocellulosic alternatives for the production of biofuels is equally important. Most commonly, biofuels are produced using a crop such as corn or soybeans to feed sugars to the yeast, Saccharomyces cerevisiae for the fermentation of ethanol. Lignocellulosic biofuel production would eliminate the need for food crops and transition to biomass such as switchgrass, poplar, or corn stover. Currently, lignocellulosic biofuel production is limited primarily because of the cost of converting the biomass to fermentable sugars than can then be metabolized by yeast. To overcome this barrier, a process must be employed that can convert lignocellulosic biomass directly to fuels and chemicals quickly and affordably. Clostridium thermocellum is one of the most promising candidates for the production of advanced biofuels because of its potential ability to convert cellulose directly to ethanol without the expensive addition of enzymes. Challenges to implementing C. thermocellum on an industrial scale still exist including side product formation, slow growth, limited titers, inhibition on high solids loadings, and a limited ability to perform genetic engineering. This thesis considers all of these concerns with C. thermocellum and attempts to systematically improve each characteristic to produce an industrially relevant strain of C. thermocellum for advanced biofuel production. Metabolic engineering is applied for the elimination of undesirable fermentation products. Laboratory evolution and medium supplementation are used to improve and understand the mechanisms that influence growth rate, and systematic approaches are used to improve transformation for more efficient genetic engineering of C. thermocellum in the future.


Improving Metabolic Engineering and Characterization of Clostridium Thermocellum for Improved Cellulosic Ethanol Production
Language: en
Pages: 91
Authors: Beth Alexandra Papanek
Categories: Biomass energy
Type: BOOK - Published: 2016 - Publisher:

GET EBOOK

Biofules are an important option for humanity to move away from its dependence on fossil fuels. Transitioning from food crops to lignocellulosic alternatives fo
Model-guided Systems Metabolic Engineering of Clostridium Thermocellum
Language: en
Pages:
Authors: Christopher Mark Gowen
Categories: Biomass energy
Type: BOOK - Published: 2011 - Publisher:

GET EBOOK

Metabolic engineering of microorganisms for chemical production involves the coordination of regulatory, kinetic, and thermodynamic parameters within the contex
Metabolic Engineering
Language: en
Pages: 1075
Authors: Sang Yup Lee
Categories: Science
Type: BOOK - Published: 2021-06-02 - Publisher: John Wiley & Sons

GET EBOOK

Learn more about foundational and advanced topics in metabolic engineering in this comprehensive resource edited by leaders in the field Metabolic Engineering:
Plant Biotechnology: Progress in Genomic Era
Language: en
Pages: 670
Authors: S. M. Paul Khurana
Categories: Science
Type: BOOK - Published: 2019-11-14 - Publisher: Springer Nature

GET EBOOK

Refinement in sequencing technologies and potential of genomic research resulted in meteoric growth of biological information such as sequences of DNA, RNA and
Biofuels
Language: en
Pages: 294
Authors: Jonathan R. Mielenz
Categories: Science
Type: BOOK - Published: 2012-03-07 - Publisher: Humana Press

GET EBOOK

This editor believes the public finally understands that bioenergy should be a critical new 39 component of our world’s future energy supply. While this new e