Fast Image Restoration In Light Sheet Fluorescence Microscopy With Extended Depth Of Field Using Gpus
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Fast Image Restoration in Light-sheet Fluorescence Microscopy with Extended Depth of Field Using GPUs
Author | : David Castillo Andreo |
Publisher | : |
Total Pages | : |
Release | : 2015 |
Genre | : |
ISBN | : |
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Light sheet fluorescence microscopy (LSFM) is used in many biological research experiments that require fast three-dimensional (3D) imaging up to a few volumes per second. Wavefront coding (WFC) microscopy provides LSFM with 3D real-time imaging capabilities introducing a controlled aberration that extends the depth of field (DOF) of the microscope objective. Resulting images, however, are blurred and need to be processed to recover the original sharpness in a CPU-intensive deconvolution which makes real-time visualization unattainable. We present computational tools based on GPU parallel computing to achieve real-time deconvolution and visualization of the images obtained with WFC light-sheet microscopy.
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