Metabolic Engineering Of Cellulolytic Clostridium Cellulovorans For Biofuel Production Directly From Cellulosic Biomass

In summary, C. cellulovorans was metabolically engineered to produce n-butanol and ethanol directly from cellulosic biomass, with the development of its transformation method for the first time. In addition, the engineered C. cellulovorans could produce 1.6 g/L n-butanol directly from cellulose, which is the highest, compared to other wild-type and engineered cellulolytic strains. This project provided a promising platform for the production of biofuel and other value-added products directly from lignocellulosic biomass.