Improving Metabolic Engineering and Characterization of Clostridium Thermocellum for Improved Cellulosic Ethanol Production

Improving Metabolic Engineering and Characterization of Clostridium Thermocellum for Improved Cellulosic Ethanol Production
Author: Beth Alexandra Papanek
Publisher:
Total Pages: 91
Release: 2016
Genre: Biomass energy
ISBN:


Download Improving Metabolic Engineering and Characterization of Clostridium Thermocellum for Improved Cellulosic Ethanol Production Book in PDF, Epub and Kindle

Biofules are an important option for humanity to move away from its dependence on fossil fuels. Transitioning from food crops to lignocellulosic alternatives for the production of biofuels is equally important. Most commonly, biofuels are produced using a crop such as corn or soybeans to feed sugars to the yeast, Saccharomyces cerevisiae for the fermentation of ethanol. Lignocellulosic biofuel production would eliminate the need for food crops and transition to biomass such as switchgrass, poplar, or corn stover. Currently, lignocellulosic biofuel production is limited primarily because of the cost of converting the biomass to fermentable sugars than can then be metabolized by yeast. To overcome this barrier, a process must be employed that can convert lignocellulosic biomass directly to fuels and chemicals quickly and affordably. Clostridium thermocellum is one of the most promising candidates for the production of advanced biofuels because of its potential ability to convert cellulose directly to ethanol without the expensive addition of enzymes. Challenges to implementing C. thermocellum on an industrial scale still exist including side product formation, slow growth, limited titers, inhibition on high solids loadings, and a limited ability to perform genetic engineering. This thesis considers all of these concerns with C. thermocellum and attempts to systematically improve each characteristic to produce an industrially relevant strain of C. thermocellum for advanced biofuel production. Metabolic engineering is applied for the elimination of undesirable fermentation products. Laboratory evolution and medium supplementation are used to improve and understand the mechanisms that influence growth rate, and systematic approaches are used to improve transformation for more efficient genetic engineering of C. thermocellum in the future.


Improving Metabolic Engineering and Characterization of Clostridium Thermocellum for Improved Cellulosic Ethanol Production
Language: en
Pages: 91
Authors: Beth Alexandra Papanek
Categories: Biomass energy
Type: BOOK - Published: 2016 - Publisher:

GET EBOOK

Biofules are an important option for humanity to move away from its dependence on fossil fuels. Transitioning from food crops to lignocellulosic alternatives fo
Model-guided Systems Metabolic Engineering of Clostridium Thermocellum
Language: en
Pages:
Authors: Christopher Mark Gowen
Categories: Biomass energy
Type: BOOK - Published: 2011 - Publisher:

GET EBOOK

Metabolic engineering of microorganisms for chemical production involves the coordination of regulatory, kinetic, and thermodynamic parameters within the contex
Towards Increasing Lignocellulose to Biofuel Conversion by Clostridium Thermocellum
Language: en
Pages: 0
Authors: Alan Froese
Categories:
Type: BOOK - Published: 2021 - Publisher:

GET EBOOK

Alternative low-carbon transportation fuels, such as biofuels, are needed to replace or supplement fossil fuels in order to lower global greenhouse gas emission
Elimination of Metabolic Pathways to All Traditional Fermentation Products Increases Ethanol Yields in Clostridium Thermocellum
Language: en
Pages: 6
Authors:
Categories:
Type: BOOK - Published: 2015 - Publisher:

GET EBOOK

Clostridium thermocellum has the natural ability to convert cellulose to ethanol, making it a promising candidate for consolidated bioprocessing (CBP) of cellul
Metabolic Modeling of Clostridia for Biofuel Production
Language: en
Pages:
Authors: Satyakam Dash
Categories:
Type: BOOK - Published: 2019 - Publisher:

GET EBOOK

Anaerobic Clostridium spp. is an important microbial bio-production host that can producea range of solvents and utilize a broad spectrum of substrates includin