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G Protein Signaling
Author | : Alan V. Smrcka |
Publisher | : Springer Science & Business Media |
Total Pages | : 246 |
Release | : 2008-02-02 |
Genre | : Science |
ISBN | : 1592594301 |
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Alan V. Smrcka presents a collection of cutting-edge methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. Among the readily reproducible techniques presented are those for the purification of G proteins and effectors enzymes, assays of these purified G proteins and effector enzymes, and for the study of G proteins interactions with effectors in intact cells. Additional methods are provided for assaying G protein coupled receptor structure, function, and localization, and for studying the physiological roles for endogenous G proteins.
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